Abstract: After the usage of the Total Scanning Fluorescence (TSF) index on the Central Uplift of the South Yellow Sea Basin, more parameters, such as TSF, alkane and aromatic hydrocarbon were measured for the North Depression. The maximum TSF Fluorescence Intensity (MFI) excitation wavelengths is 227nm -233nm and the emission wavelengths 324-337 nm, while the sub-MFI excitation is 257-260 nm and emission 357-361 nm, both of which show similar patterns with those of the Central Uplift. The starting threshold for screening indicator of MFI, T/D and aromatic hydrocarbon percentage content determined by frequency histogram is 800 units, 2.5 and thirteen percent respectively. With the distribution pattern of the index, nine comprehensive anomalous areas can be determined. Based on the synthetic judgement of anomalous index conformity, structure matching and source rock development, the areas can be classified into four ranks according to the reliability of the anomalies.